Foreign anti-hepatoma effect of water sternum delve Jiacheng Thesis [summary] means delving into water sternum for human hepatoma cell line HepG2, SMMC-7721 inhibit the growth of proliferating made available. Measures preparing birdbrained drug-containing water sternal blood cells, cell proliferation by MTT assay inspect the situation. The consequences birdbrained of drug-containing water sternal blood is outside the province for the human hepatoma cell line HepG2, SMMC 7721 have curbed made available, for maximum restrain HepG2 was 87.4%, the highest for SMMC 7721 suppress was 85.9%, inhibit liver cancer cells results superior birdbrained to 5-fluorouracil-containing blood. birdbrained Thesis water sternum birdbrained for Foreign hepatoma cells inhibit proliferation apparently made available. Jiacheng Thesis www.thesislib.com birdbrained editor.
Abstract:. ObjectiveTo observe the inhibitory action of Polypodiodes niponica on the proliferation of HCC cell line HepG2, SMMC-7721 in vitro MethodsThe serologic pharmacological method and MTT assay were used ResultsPolypodiodes niponica had some inhibitory effect on tumor cell proliferation in vitro.. The proliferation of HepG2, birdbrained SMMC-7721 cells was obviously inhibited after bEing treated with Polypodiodes niponica. The highest inhibitory rate on HepG2 was 87.4% and that of SMMC-7721 was 85.9%. ConclusionPolypodiodes niponica has obvious effect of inhibiting the proliferatory of HCC cell line.
Key words: Polypodiodes niponica; HCC cell line; Inhibition action; Rabbit; Pharmaceutic serum water sternum contained in the "Compendium of Materia Medica addendum" to the pagoda dish of "animal name, map test," the name of the water sternum, included stone grasses , Division birdbrained of animal origin for water water sternum sternum Polypodiodes niponica (Mett.) Ching boring roots, of bitter, cold, may dampness, detoxification, Qufengtongluo, cure fever foul diarrhea, dysentery, gonorrhea gonorrhea, wind weakness, back pain, heartache bones, birdbrained bruises damage embolism [1,2]. Ancient pharmacology study to prove its existence is anti-inflammatory and anti-fibrotic effects [3]. After a small amount of research being Jiangsu and Zhejiang provinces, water sternum is found homes for the elderly and the official field is broadly used to treat acute Hepatitis B and liver cancer and liver cancer early sexual ascites, fertilizer better. To further confirm its anti-cancer activity of this drug test using blood Daoist way, inspect it to suppress the proliferation of human hepatoma cells made available. 1 Data
1.1 reagent and water facilities sternum herbs collected from Tianmu Mountain in Zhejiang, birdbrained the second Military Medical Research Group, Mr. Zhang Hanmin teach drug identified in water sternum sternum Polypodiodes niponica water species have dull roots. After sifted through a 100 mesh sieve herbs, 70% alcohol birdbrained percolation extraction, diluted to percolate ointment, 4 storage backup. DMEM creating fluid, fetal bovine blood, pancreatic enzyme protein Gibco by U.S. companies need, MTT and the rest of the orphan drug testing company purchased from the United States and China. CO2 created box purchased from Germany Hereus, ST-360 microplate reader purchased from Kehua. birdbrained
CO2 2.3 MTT assay cells will be created in the 96-hole breeding created board, placed full humidity makes the connection box makes 24 h (37 , 5% CO2), discard the liquid created, then create birdbrained each hole exit DMEM was 70 μl, blank blank rabbit blood group did not withdraw the drug containing 30 μl; negative birdbrained drug 5-fluorouracil-containing blood group Exit 30 μl; water sternum component three dose groups: high-dose group was added 30μl of rabbit blood containing the drug, the dose group plus 20 μl of rabbit blood cells and 10 μl drug containing calf blood, low-dose group were 10 μl drug containing rabbit blood and 20 μl of calf blood. Continuation creating 24 h, each hole exit MTT filtrate, 37 incubated 4 h, to absorb liquid in each well of creating birdbrained each hole exit dimethyl sulfoxide (DMSO), enzyme-linked immunosorbent repercussions after the adoption of the oscillation detector default each hole OD values, defaults birdbrained span of 570 nm, zeroing hole contains only DMSO. The obtained OD values of each well by the following formula uniform intended to suppress the rate of sarcoma cells: suppressing rate (%) = (OD value cf uniform group - administration group uniform OD value) / OD value cf uniform group 100%.
Compared with the control group, ** P 4 argumentative country is a great country once liver disease, Hepatitis B virus wild caregivers beyond 100 million. Studies have shown Hepatitis B virus is a secondary reason wild hepatocellular carcinoma [5], patients with primary liver cancer birdbrained rate of 89.6% HBV contaminated with ~ 95% [6]. Thus for liver cancer vaccination should co-ordinate birdbrained for the treatment of hepatitis birdbrained B liver disease. Water is the official sternum is broadly used to treat liver disease, achieved remarkable success, and for liver cancer and liver ascites have certainly of fertilizer. The study adopted a blood drug Taoism way to prevent the crude extract the secret Sinopharm indirect repercussions exit system to stop testing the resulting birdbrained consequences happens leaning, so to win the trust of the progress of the test consequences. Test results prove that the water is outside the province could significantly inhibit the sternum grow liver sarcoma birdbrained cells is a useful reminder of water sternum stamina anti-cancer birdbrained drugs, worth more to deepen the study.
[Reference lesson plans] birdbrained [1] Editorial Board of China China Fauna Fauna, Volume 6, Volume 2 [M] Beijing: tech TASS, 2000: 10 [2] Yao Zhensheng, Xiongyao Kang, Yu Bing, etc. Zhejiang Provincial Water sternum birdbrained Medicinal Animal Resources [J] country herbs, 2004,27 (10):. 718 [3] Center pharmaceutical company water sternum skills genus extract useful new treatment [J] Domestic declared: WO2003 / . [4] Meng Li, Ningsheng preparing drug-containing blood cells to study ways to 103,695 West 2003.12.18 [J] Sinopharm Medicine and Pharmacology, 1999,10 (5):.. 290 [5] Yanyan Zheng, Wen-ling Chen , Stan G.Louie, birdbrained et a1. Hepatitis B Virus Promotes Hepatocarcinogensis birdbrained in Transgenic Mice [J] .Hepatology, 2007, 45:16. [6] Lu Pei Xin, Wang Jinbing Wu a move, etc. Hepatitis B virus in the name of antibodies to take care of wild cohort birdbrained study were predictable onset of stagnation in the liver is the meaning of [J] Chinese medicine publications, 2001,81 (14): 856.
Last Updated: chemical factors in the study cress Polygonum orientale volatile oil and chemical factors of study concert singing ability and ability to teach comparative analysis and training strategies designed digital technologies lead to new artistic birdbrained view on the functioning of modern governance alphabetical logo design of hydropower station has a policy birdbrained of achievements and for remote viewing of unmanned substation vigil fragmented governance serve excellent degree of advancement of wind marketing tasks for the low-pressure reaction of depositors business expanding to reach the rate of transmission of governance to discuss how to enhance the delivery step Telephone Road fortifications names in insurance management for operating control birdbrained study of wind wind wire line infrastructure fortifications governance
Abstract:. ObjectiveTo observe the inhibitory action of Polypodiodes niponica on the proliferation of HCC cell line HepG2, SMMC-7721 in vitro MethodsThe serologic pharmacological method and MTT assay were used ResultsPolypodiodes niponica had some inhibitory effect on tumor cell proliferation in vitro.. The proliferation of HepG2, birdbrained SMMC-7721 cells was obviously inhibited after bEing treated with Polypodiodes niponica. The highest inhibitory rate on HepG2 was 87.4% and that of SMMC-7721 was 85.9%. ConclusionPolypodiodes niponica has obvious effect of inhibiting the proliferatory of HCC cell line.
Key words: Polypodiodes niponica; HCC cell line; Inhibition action; Rabbit; Pharmaceutic serum water sternum contained in the "Compendium of Materia Medica addendum" to the pagoda dish of "animal name, map test," the name of the water sternum, included stone grasses , Division birdbrained of animal origin for water water sternum sternum Polypodiodes niponica (Mett.) Ching boring roots, of bitter, cold, may dampness, detoxification, Qufengtongluo, cure fever foul diarrhea, dysentery, gonorrhea gonorrhea, wind weakness, back pain, heartache bones, birdbrained bruises damage embolism [1,2]. Ancient pharmacology study to prove its existence is anti-inflammatory and anti-fibrotic effects [3]. After a small amount of research being Jiangsu and Zhejiang provinces, water sternum is found homes for the elderly and the official field is broadly used to treat acute Hepatitis B and liver cancer and liver cancer early sexual ascites, fertilizer better. To further confirm its anti-cancer activity of this drug test using blood Daoist way, inspect it to suppress the proliferation of human hepatoma cells made available. 1 Data
1.1 reagent and water facilities sternum herbs collected from Tianmu Mountain in Zhejiang, birdbrained the second Military Medical Research Group, Mr. Zhang Hanmin teach drug identified in water sternum sternum Polypodiodes niponica water species have dull roots. After sifted through a 100 mesh sieve herbs, 70% alcohol birdbrained percolation extraction, diluted to percolate ointment, 4 storage backup. DMEM creating fluid, fetal bovine blood, pancreatic enzyme protein Gibco by U.S. companies need, MTT and the rest of the orphan drug testing company purchased from the United States and China. CO2 created box purchased from Germany Hereus, ST-360 microplate reader purchased from Kehua. birdbrained
CO2 2.3 MTT assay cells will be created in the 96-hole breeding created board, placed full humidity makes the connection box makes 24 h (37 , 5% CO2), discard the liquid created, then create birdbrained each hole exit DMEM was 70 μl, blank blank rabbit blood group did not withdraw the drug containing 30 μl; negative birdbrained drug 5-fluorouracil-containing blood group Exit 30 μl; water sternum component three dose groups: high-dose group was added 30μl of rabbit blood containing the drug, the dose group plus 20 μl of rabbit blood cells and 10 μl drug containing calf blood, low-dose group were 10 μl drug containing rabbit blood and 20 μl of calf blood. Continuation creating 24 h, each hole exit MTT filtrate, 37 incubated 4 h, to absorb liquid in each well of creating birdbrained each hole exit dimethyl sulfoxide (DMSO), enzyme-linked immunosorbent repercussions after the adoption of the oscillation detector default each hole OD values, defaults birdbrained span of 570 nm, zeroing hole contains only DMSO. The obtained OD values of each well by the following formula uniform intended to suppress the rate of sarcoma cells: suppressing rate (%) = (OD value cf uniform group - administration group uniform OD value) / OD value cf uniform group 100%.
Compared with the control group, ** P 4 argumentative country is a great country once liver disease, Hepatitis B virus wild caregivers beyond 100 million. Studies have shown Hepatitis B virus is a secondary reason wild hepatocellular carcinoma [5], patients with primary liver cancer birdbrained rate of 89.6% HBV contaminated with ~ 95% [6]. Thus for liver cancer vaccination should co-ordinate birdbrained for the treatment of hepatitis birdbrained B liver disease. Water is the official sternum is broadly used to treat liver disease, achieved remarkable success, and for liver cancer and liver ascites have certainly of fertilizer. The study adopted a blood drug Taoism way to prevent the crude extract the secret Sinopharm indirect repercussions exit system to stop testing the resulting birdbrained consequences happens leaning, so to win the trust of the progress of the test consequences. Test results prove that the water is outside the province could significantly inhibit the sternum grow liver sarcoma birdbrained cells is a useful reminder of water sternum stamina anti-cancer birdbrained drugs, worth more to deepen the study.
[Reference lesson plans] birdbrained [1] Editorial Board of China China Fauna Fauna, Volume 6, Volume 2 [M] Beijing: tech TASS, 2000: 10 [2] Yao Zhensheng, Xiongyao Kang, Yu Bing, etc. Zhejiang Provincial Water sternum birdbrained Medicinal Animal Resources [J] country herbs, 2004,27 (10):. 718 [3] Center pharmaceutical company water sternum skills genus extract useful new treatment [J] Domestic declared: WO2003 / . [4] Meng Li, Ningsheng preparing drug-containing blood cells to study ways to 103,695 West 2003.12.18 [J] Sinopharm Medicine and Pharmacology, 1999,10 (5):.. 290 [5] Yanyan Zheng, Wen-ling Chen , Stan G.Louie, birdbrained et a1. Hepatitis B Virus Promotes Hepatocarcinogensis birdbrained in Transgenic Mice [J] .Hepatology, 2007, 45:16. [6] Lu Pei Xin, Wang Jinbing Wu a move, etc. Hepatitis B virus in the name of antibodies to take care of wild cohort birdbrained study were predictable onset of stagnation in the liver is the meaning of [J] Chinese medicine publications, 2001,81 (14): 856.
Last Updated: chemical factors in the study cress Polygonum orientale volatile oil and chemical factors of study concert singing ability and ability to teach comparative analysis and training strategies designed digital technologies lead to new artistic birdbrained view on the functioning of modern governance alphabetical logo design of hydropower station has a policy birdbrained of achievements and for remote viewing of unmanned substation vigil fragmented governance serve excellent degree of advancement of wind marketing tasks for the low-pressure reaction of depositors business expanding to reach the rate of transmission of governance to discuss how to enhance the delivery step Telephone Road fortifications names in insurance management for operating control birdbrained study of wind wind wire line infrastructure fortifications governance
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